Genotypic analysis of Bacillus thuringiensis serovars by RAPD-PCR

Authors

  • Jeane Q. Chaves
  • Fernanda A. Stringuini
  • Eder S. Pires
  • Clara F.G. Cavados

DOI:

https://doi.org/10.4013/4755

Abstract

In this study, genomic DNA fingerprints of 40 Bacillus thuringiensis serovars representing different serotypes were obtained by Randomic Amplifi cation of Polymorphic DNA (RAPDPCR) using three different randomic primers. The electrophoretic analysis of RAPD band profiles showed the presence of polymorphism among the serovars and serotypes. Between the primers used in this study, primer 2 was the most discriminatory and, therefore, was used to build the dendrogram. The similarity analysis of the electrophoretic profiles showed low level of similarity (16%) among the different B. thuringiensis serovars. The serovars were divided into two main groups, and these indicated a high level of genetic divergence both intra and inter-group. Results suggested that genomic fingerprints obtained by RAPD can be used for the genotypic characterization and identification of B. thuringiensis serovars as a complement to flagellar serology.

Key words: Bacillus thuringiensis, RAPD-PCR, genetic similarity, flagellar serology.

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Published

2021-06-15

Issue

Vol. 5 No. 2 (2010): May/August

Section

Articles

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